Stimulation of proliferation and immunoglobulin production of human-human hybridoma by various types of caseins and their protease digests |
| |
Authors: | Koji Yamada Ichiro Ikeda Hiroto Nakajima Sanetaka Shirahata Hiroki Murakami |
| |
Affiliation: | (1) Department of Food Science and Technology, Kyushu University 46-09, 6-10-1 Hakozaki, Higashi-ku, 812 Fukuoka, Japan;(2) Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University 46-09, 6-10-1 Hakozaki, Higashi-ku, 812 Fukuoka, Japan |
| |
Abstract: | We have studied the effect of such milk proteins as caseins, lactalbumin, and lactoglobulin, on proliferation and immunoglobulin production of human-human hybridoma HB4C5 cells. It was found that -, -, and -caseins stimulated both proliferation and IgM product ion of human-human hybridoma HB4C5 cells, while the activities of -lactalbumin and -lactoglobulin were negligible. To localize the active sites of these caseins, effect of protease treatments on the activities were examined. When caseins were digested with trypsin, casein digests stimulated proliferation of the hybridoma, but not their IgM production. When -casein was digested with chymosin and fractionated top--casein and glycomacropeptide, both fragments stimulated proliferation of the cells, but onlyp--casein fragment stimulated IgM production. These results indicate that -casein has at least two proliferation stimulating sites and an IgM production stimulating site in thep--casein region. |
| |
Keywords: | immunoglobulin production stimulating factor p- /content/q7j75132n406649r/xxlarge954.gif" alt=" kappa" align=" BASELINE" BORDER=" 0" >-casein serum-free culture human-human hydridoma |
本文献已被 SpringerLink 等数据库收录! |
|