Control of Morphogenesis in Sorghum by 2,4-Dichlorophenoxyacetic Acid and Cytokinins |
| |
Authors: | BHASKARAN, SHYAMALA SMITH, ROBERTA H. |
| |
Affiliation: | Department of Soil and Crop Sciences, Texas A&M University College Station, TX 77843, USA |
| |
Abstract: | 2, 4-Dichlorophenoxyacetic acid caused a shortening of rootsand shoots when mature seeds of Sorghum bicolor (L.) Moenchcv. BT3197 were germinated on Murashige and Skoog (MS) mediumthat contained 2,4-D. Shoot growth was restored with cytokinins.A callus formed at the nodal region, the further differentiationof which was determined by the ratio of 2,4-D and cytokininsin the initial culture medium. A high auxin to cytokinin ratiopromoted primarily root differentiation while a high cytokininto auxin ratio promoted multiple bud development. Isolated shootapical meristem with the subtending node produced embryogeniccallus at low cytokinin levels and green buds on high cytokininlevels when cultured in the presence of 2,4-D. It is concludedthat cells potentially capable of differentiation into roots,somatic embryos or axillary buds are present in the first nodalregion. Sorghum bicolor, organogenesis, embryogenesis, 2, 4-D: cytokinin ratios, tissue culture |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|