| 重组人碱性成纤维细胞生长因子基因工程菌的高密度培养 |
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| 引用本文： | 沈林南,魏东芝.重组人碱性成纤维细胞生长因子基因工程菌的高密度培养[J].微生物学杂志,1999,19(4):11-14. |
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| 作者姓名： | 沈林南 魏东芝 |
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| 作者单位： | 华东理工大学生物化学研究所!上海200237 |
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| 摘 要： | 根据重组人碱性成纤维细胞生长因子基因工程菌的生长特点，对其高密度发酵工艺条件进行研究和改进，采用“平衡DO-State”控制策略进行分批补料培养中的葡萄糖流加，有效地控制了培养过程中代谢副产物-乙酸的产生及其对工程菌生长的抑制作用，使发酵终了时乙酸浓度由15．6g/L下降为2.6g/L，而菌体密度则由15．2gDCW/L提高到30．2gDCW/L。
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| 关 键 词： | 人碱性成纤维细胞生长因子 重组大肠杆菌 高密度培养 |
HIGH CELL DENSITY CULTIVATION OF RECOMBINANT Escherichia Coli EXPRESSING HUMAN BASIC FIBROBLAST GROWTH FACTOR |
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| Shen Linnan et al..HIGH CELL DENSITY CULTIVATION OF RECOMBINANT Escherichia Coli EXPRESSING HUMAN BASIC FIBROBLAST GROWTH FACTOR[J].Journal of Microbiology,1999,19(4):11-14. |
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| Authors: | Shen Linnan |
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| Abstract: | According to the growing characteristics Of recominant E. coli expressing human basic fibroblast growth factor, a method of cultivation and its conditions in high cell density was studied, adopting balanced DO - State control strategy for batch feeding cultivation to prevent from acetic acid excretion. Consequently, acetic acid concentration in the mediurn was reduced from 15. 6g/L to 2. 6g/L. And the cell concentration was increased from 15. 2gDCW/L upto 30. 3gDCW/L. |
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| Keywords: | human basic fibroblast growth factor recombinant E coli high cell densitycultivation |
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