Rapid purification of bacterial plasmids and coliphage M13 RF without CsCl centrifugation |
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| Authors: | D S Holmes |
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| Affiliation: | Department of Biological Sciences, State University of New York at Albany, 1400 Washington Avenue, Albany, New York 12222 USA |
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| Abstract: | A simple and rapid procedure for the purification of plasmids from Escherichia coli K12 has been developed. Bacterial cells are subjected to the boiling procedure [D. S. Holmes, and M. Quigley Anal. Biochem.114, 193–197 (1981)] followed by removal of contaminating RNA by chromatography on Sepharose 2B and of genomic DNA by acid-phenol extraction. Plasmids are recovered with good yield. They can be restricted and ligated and will transform host cells. A simple modification of the procedure allows it to be used for the isolation of coliphage M13 RF DNA. |
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| Keywords: | To whom correspondence should be addressed at Building 37 Room 2B26. |
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