Nigericin forms highly stable complexes with lithium and cesium |
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Authors: | Raúl Alva J Antonio Lugo-R Eduardo Arzt Jorge Cerbón Blanca E Rivera Mireya Toro Sergio Estrada-O |
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Institution: | (1) Departamento de Ciencias de la Salud, División de Ciencias Biologicas y de la Salud, Universidad Autónoma Metropolitanalztapalapa, A.P. 55-535, 09340 México 13, D.F., México;(2) Present address: Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina;(3) Departamento de Bioquímica, Centro de Investigación y de Estudios Avanzados de I.P.N., México, D.F., México |
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Abstract: | Nigericin is a monocarboxylic polyether molecule described as a mobile K+ ionophore unable to transport Li+ and Cs+ across natural or artificial membranes. This paper shows that the ion carrier molecule forms complexes of equivalent energy demands with Li+, Cs+, Na+, Rb+, and K+. This is in accordance with the similar values of the complex stability constants obtained from nigericin with the five alkali metal cations assayed. On the other hand, nigericinalkali metal cation binding isotherms show faster rates for Li+ and Cs+ than for Na+, K+, and Rb+, in conditions where the carboxylic proton does not dissociate. Furthermore, proton NMR spectra of nigericin-Li+ and nigericin-Cs+ complexes show wide broadenings, suggesting strong cation interaction with the ionophore; in contrast, the complexes with Na+, K+, and Rb+ show only clear-cut chemical shifts. These latter results support the view that nigericin forms highly stable complexes with Li+ and Cs+ and contribute to the explanation for the inability of this ionophore to transport the former cations in conditions where it catalyzes a fast transport of K+>Rb+>Na+.Part of the results of this paper were presented at the 14th International Congress of Biochemistry in Prague, Czechoslovakia. |
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Keywords: | Nigericin carboxylic polyether carboxylic ionophore ion transport ionic complexes |
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