| Abstract: | Nine strains of an adenovirus serotype were recovered in bovine testicle cell cultures from Japanese cattle suffering with an acute febrile illness accompanied by rhinorrhea and diarrhea. The isolated virus was shown to have the physicochemical properties of the adenovirus group such as the nucleic acid type, the size and ultrastructure of the virion, and the resistance to ether and chloroform. The isolated virus produced eosinophilic intranuclear inclusion bodies characteristic of adenoviruses and the group specific antigen of adenovirus in bovine testicle cell culture. According to the results of cross-neutralization tests the isolated virus represents a serological type distinct from bovine adenovirus types 1, 2 and 3 and from the Nagano virus. The isolated virus agglutinated erythrocytes of cattle, sheep, goat, guinea pig, rat, hamster and mouse, but not those of vervet monkey, horse, goose and chicken. HI test using cattle erythrocytes corroborated the results of serological typing by neutralization tests. |
