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Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae |
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| Authors: | Amy Rosenbluh M. Mevarech Yigal Koltin Jessica A. Gorman |
| Affiliation: | (1) Department of Microbiology, G.S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv, Israel;(2) Department of Molecular Genetics, Research and Development, Smith Kline and French Laboratories, 1500 Spring Garden Street, 19101 Philadelphia, PA, USA |
| Abstract: | Summary A genomic library of the asexual pathogenic yeast Candida albicans was constructed in the S. cerevisiae vector YEp13. The library contains a representation of the entire genome with a probability of 99%. The expression of the genes of C. albicans in S. cerevisiae was examined and two mutations his3-1 and trp1-289 of S. cerevisiae were complemented by the cloned genes of C. albicans. The hybridization data indicates that the plasmids complementing the mutations of S. cerevisiae contain sequences from C. albicans. |
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