维生素E和微量元素Se对人肝癌细胞生长、分化及其癌基因表达的影响

本工作观察了体外环境中不同水平的维生素Ｅ和微量元素Ｓｅ对人肝癌细胞株（ＳＭＭＣ－７７２１）生长、分化和其癌基因（Ｎ－ｒａｓ、ｃ－ｍｙｃ）表达水平的影响。实验结果表明：高水平维生素Ｅ（２．４、９．２、２４．０ｎｍｏｌ／Ｌ）和Ｓｅ（０．１５、０．３０、０．６０ｎｍｏｌ／Ｌ）对肝癌细胞的集落形成率具有明显的抑制作用；生化分析显示高水平维生素Ｅ和微量元素Ｓｅ均可明显抑制环境中脂质过氧化的水平，Ｓｅ对癌细胞甲胎蛋白的分泌有明显的抑制作用，而维生素Ｅ作用不明显。细胞原位杂交发现维生素Ｅ浓度为２．４和９．２ｎｍｏ１／Ｌ时对细胞癌基因Ｎ－ｒａｓ的表达具有明显抑制作用；Ｓｅ浓度为０．１５和０．３０ｎｍｏｌ／Ｌ时对癌基因ｃ－ｍｙｃ的表达明显抑制。实验还观察了维生素Ｅ和Ｓｅ之间的叠加效应，结果显示除对环境中脂质过氧化的抑制作用具有叠加效果外，对其他指标没有明显作用。

EFFECT OF VITAMIN E AND SELENIUM ON THEGROWTH,DIFFERENTIATION AND ONCOGENIC EXPRESSION IN HUMAN HEPATOMA CELL LINE SMMC-7721

In the present study,the effects of vitamin E and selenium at different concentrations in cul-ture medium on the growth,differentiation and oncogenic expression in human hepatoma cell line SMMC-7721 were observed. The results showed that high concentration of vitamin E(2.4,9.2and 24.0nmol / L) and selenium(0.15,0.30 and 0.60 nmol/L)in culture medium could inhib-it the growth of human hepatoma cells,and suppress the lipid peroxidation of the systemsignificantly. Vitamin E had no effect on the cellular secretion of alpha fetoprotein(AFP), butselenium could inhibit it. The cellular mRNA expression of c-myc and N-ras oncogenes identi-fied by hybridization in situ followed by image analyzer indicated that vitamin E(2.4 and 9.2nmol / L) could suppress the expression of cellular N-ras oncogene but not c-myc, whileselenium(0.15 and 0.30 nmol / L) could inhibit the expression of cellular c-myc oncogene butnot N-ras.Observation on the synergistic effects of vitamin E and selenium showed that they hadno such effects on the cellular growth,secretion of AFP and expression of oncogenes exceptsynergistic inhibitory effect on the lipid peroxidation of the system.