Glycosphingolipid biosynthesis during myogenesis of rat L6 cells in vitro |
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Authors: | Kenneth C Leskawa Robert E Erwin Paul E Buse Edward L Hogan |
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Institution: | (1) Department of Neurology, The Medical University of South Carolina, 171 Ashley Avenue, SC 29425 Charleston, USA;(2) Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, 40292 Louisville, KY, USA |
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Abstract: | Summary Glycosphingolipid biosynthesis was examined using 3H]-galactose as a precursor as rat L6 myoblasts fused to form multinucleated myotubes. Incorporation of label into neutral glycolipids decreased steadily as the population of myotubes increased, so that final biosynthesis was one-half that observed with myoblasts (p < 0.02). Conversely, ganglioside biosynthesis doubled during myoblast confluency (p < 0.02) and then decreased as myotubes formed. Qualitatively, L6 cells synthesized large amounts of ganglioside GM3 during all myogenic phases. The major neutral glycosphingolipid products were lactosylceramide and paragloboside (nLcOse4Cer). Few changes in TLC autoradiographic patterns were noted during differentiation, with the exception of a slight decrease in ganglioside GM1. The results indicate that the biosynthesis of glycosphingolipids is tightly regulated during myogenesis in vitro and suggest a role for membrane gangliosides in muscle cell differentiation.Abbreviations GM1
II3NeuAc-GgOse4Cer
- GM3
II3NeuAc-GgOse2Cer
- MG4
IV3NeuAc-nLcOse4Cer
- MG6
VI3NeuAc V4Gal-IV3GlcNAc-nLcOse4Cer
- TLC
Thin-Layer Chromatography
- DMEM
Dulbecco's Modified Eagles' Medium |
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Keywords: | muscle cells myogenesis myoblasts differentiation glycosphingolipids gangliosides |
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