Prostaglandin endoperoxides 21. Covalent binding of levuglandin E2 with proteins |
| |
| Authors: | R G Salomon M R Jirousek S Ghosh R B Sharma |
| |
| Affiliation: | Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-2699. |
| |
| Abstract: | Levuglandin E2 (LGE2), a gamma-ketoaldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis, binds covalently with ram seminal vesicle microsomes. Totally synthetic 5,6-ditritio-LGE2 was prepared and used to determine that rapid covalent binding of LGE2 (initially 800 microM) occurs with 6.4 microM bovine serum albumin (greater than 10 equiv within 1 min) which approaches saturation (approximately 16 equiv) after 40 min at 37 degrees C. |
| |
| Keywords: | |
|
|
