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The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase B
Authors:Magda Gioia  Susanna Monaco  Philippe E. Van Den Steen  Giuseppe Grasso  Stefano Marini  Christopher M. Overall  Ghislain Opdenakker
Affiliation:1 Department of Experimental Medicine and Biochemical Sciences, University of Roma Tor Vergata, Via Montpellier 1, I-00133 Rome, Italy
2 Department of Oral Biological and Medical Sciences, Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada V6T1Z3
3 Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada V6T1Z3
4 Interuniversity Consortium for the Research on the Chemistry of Metals in Biological Systems, P.za Umberto I 1, I-70100 Bari, Italy
5 Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium
6 Department of Chemistry, University of Catania, V.le A. Doria, Catania, Italy
7 Institute of Biostructure and Bioimaging, CNR, V.le A. Doria, Catania, Italy
Abstract:
Keywords:MMP, matrix metalloproteinase   MMP2, gelatinase A   MMP9, gelatinase B   CBD, collagen binding domain   MCA, (7-methoxycoumarin-4-yl)acetyl   DPA, N-3-(2,4-dinitrophenyl)-  smallcaps"  >l-2,3-diaminopropionyl   PDMS, poly(dimethylsiloxane)   DMSO, dimethyl sulfoxide   SPR, surface plasmon resonance   SPRI, surface plasmon resonance imaging   NC1 domain, noncollagenous domain   MS, mass spectrometry   EHS, Engelbreth-Holm-Swarm   LPS, lipopolysaccharide   OPD, orthophenylendiamine   PBS, phosphate-buffered saline
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