Bishydrazide glycoconjugates for lectin recognition and capture of bacterial pathogens |
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Authors: | Adak Avijit Kumar Leonov Alexei P Ding Ning Thundimadathil Jyothi Kularatne Sumith Low Philip S Wei Alexander |
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Affiliation: | Department of Chemistry, Purdue University, West Lafayette, Indiana, United States. |
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Abstract: | Bishydrazides are versatile linkers for attaching glycans to substrates for lectin binding and pathogen detection schemes. The α,ω-bishydrazides of carboxymethylated hexa(ethylene glycol) (4) can be conjugated at one end to unprotected oligosaccharides, then attached onto carrier proteins, tethered onto activated carboxyl-terminated surfaces, or functionalized with a photoactive cross-linking agent for lithographic patterning. Glycoconjugates of bishydrazide 4 can also be converted into dithiocarbamates (DTCs) by treatment with CS(2) under mild conditions, for attachment onto gold substrates. The immobilized glycans serve as recognition elements for cell-surface lectins and enable the detection and capture of bacterial pathogens such as Pseudomonas aeruginosa by their adsorption onto micropatterned substrates. A detection limit of 103 cfu/mL is demonstrated, using a recently introduced method based on optical pattern recognition. |
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