锌胁迫对铜锈环棱螺致死率、富集性、CAT和SOD活性的影响

以浙江省温州市瓯海茶山某河内的铜锈环棱螺Bellamya aeruginosa为受试生物,研究了不同浓度Zn2+胁迫下,铜锈环棱螺的致死效应、铜锈环棱螺对锌的富集性以及锌对铜锈环棱螺体内的过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性的影响.结果表明Zn2+对铜锈环棱螺具有较大的毒害影响.当Zn2+浓度达到3.0 mg/L时,就会引起个别铜锈环棱螺死亡,死亡率为2.66％.随着Zn2+浓度的增大(0.3 mg/L到2.4 mg/L)和处理时间的增加(2d到8d),除个别组外,铜锈环棱螺对锌的累积量均随之上升,具有一定的时间效应和剂量效应.铜锈环棱螺在锌胁迫下,肝胰腺的CAT和SOD活性受到较大的影响.当Zn2+浓度较低时,CAT和SOD活性均有较大的升高,但当Zn2+浓度较高时,CAT和SOD活性则受到一定的抑制.

Effect of Zinc on Lethal Rate, Accumulation, CAT and SOD Activities of Bellamya aeruginosa

Effect of zinc on lethal rate,Zn enrichment,CAT and SOD activities of Bellamya aeruginosa under Zn stress were studied.The B.aeruginosa were collected from a river in Chashan of Ouhai,Wenzhou city,Zhejiang province.The result showed that the zinc had certain toxicity to B.aeruginosa.When the Zn2+ concentration was 3.0 mg/L,a few experimental animals died,the lethal rate was 2.66%.The Zn enrichment of B.aeruginosa were increasing when the Zn2+ concentration rises from 0.3 mg/L to 2.4 mg/L and when the treating time prolongate from 2 d to 8 d except few treating group.There are certain time-response and dose-response effects.Under the Zn stress,the CAT and SOD activities in the hepatopancreas of B.aeruginosa were affected significantly.When the Zn2+ concentrations were relatively low,there are higher CAT and SOD activities of B.aeruginosa.On the contrary,when the concentrations were relatively high,the CAT and SOD activities were restrained.