Absence of mitochondrial and chloroplast DNA recombinations in Brassica napus plants regenerated from protoplasts,protoplast fusions and anther culture |
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Authors: | R J Kemhle S A Yarrow S -C Wu T L Barsby |
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Institution: | (1) Allelix Agriculture, 6850 Goreway Drive, L4V 1P1 Mississauga, Ontario, Canada;(2) Present address: Biotechnica Canada, 170, 6815-8th Street N.E., T2E 7H7 Calgary, Alberta, Canada |
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Abstract: | Summary Over 400 Brassica napus plants regenerated from individual protoplasts, from protoplast fusions and from anther culture were analysed for chloroplast and mitochondrial genome rearrangements by restriction fragment length polymorphisms. None were detected, attesting to the fidelity of the tissue culture procedures employed. In the majority of protoplast fusion products, the cytoplasmic organelles had completely sorted out at the callus stage but three regenerated plants possessed mixed parental populations of mitochondrial genomes and one regenerant contained mixed chloroplast genomes. In all four examples, the cytoplasmic genome sorted out in planta in favor of one parental type which was faithfully maternally transmitted to progeny. |
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Keywords: | Brassica Mitochondrial DNA Chloroplast DNA Protoclone Protoplast fusion Anther culture |
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