| 大麦45S和5S rDNA定位及5S rDNA伸展纤维的FISH分析 |
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| 引用本文： | 赵丽娟,李立家,覃瑞,熊怀阳,宋运淳. 大麦45S和5S rDNA定位及5S rDNA伸展纤维的FISH分析[J]. 武汉植物学研究, 2005, 23(1): 15-19 |
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| 作者姓名： | 赵丽娟 李立家 覃瑞 熊怀阳 宋运淳 |
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| 作者单位： | 1. 武汉大学生命科学学院,植物发育生物学教育部重点实验室,武汉,430072
2. 武汉大学生命科学学院,植物发育生物学教育部重点实验室,武汉,430072;中南民族大学化学与生命科学学院,生物技术国家民委重点实验室,武汉,430074 |
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| 基金项目： | 湖北省杰出青年基金资助，国家自然科学基金资助项目(30270678，30270740)。 |
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| 摘 要： | 用荧光原位杂交技术对45S和5SrDNA在大麦(Hordeum vulgare L．)有丝分裂中期染色体进行了确定分析，较强的45SrDNA信号共有2对，分别分布在大麦的第1染色体的短臂和第2染色体的长臂。而5SrDNA则只有1对杂交信号，位于第3染色体的长臂，但信号较弱。用伸展DNA纤维的荧光原位杂交(Fiber—FISH)技术测定了5SrDNA在大麦的基因组中的拷贝数，计算出5SrDNA的拷贝数约为408～416。对大麦品种中rDNA位点数目的可变性进行了讨论。
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| 关 键 词： | 大麦；rDNA ；FISH定位；DNA纤维荧光原位杂交 |
| 文章编号： | 1000-470X(2005)01-0015-05 |
| 收稿时间： | 2004-05-28 |
| 修稿时间： | 2004-07-15 |
Location of 45S and 5S rDNA on Barley Chromosomes and FISH Analysis for 5S rDNA on Extended DNA Fibers |
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| ZHAO Li-Juan,LI Li-Jia,QIN Rui,XIONG Huai-Yang,SONG Yun-Chun. Location of 45S and 5S rDNA on Barley Chromosomes and FISH Analysis for 5S rDNA on Extended DNA Fibers[J]. Journal of Wuhan Botanical Research, 2005, 23(1): 15-19 |
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| Authors: | ZHAO Li-Juan LI Li-Jia QIN Rui XIONG Huai-Yang SONG Yun-Chun |
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| Affiliation: | 1. Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China; 2. Key Laboratory of The State Ethnic Affairs Commission for Biologic Technology, College of Chemistry and Life Science, South-Center University for Nationalities, Wuhan, 430074, China |
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| Abstract: | With the help of fluorescene in situ hybridization(FISH),we have located and analyzed the sites of the 45S and 5S rDNA on the mitotic metaphase chromosomes of barley (Hordeum vulgare L.).The results indicated that there were two pairs of signs detected for 45S rDNA,each at 1S and 2L.The signal of 5S rDNA was relatively weak,and it was located on 3L.The copy number of 5S rDNA was determined by extended DNA fiber based on DNA fiber fluorescence in situ hybridization(Fiber-FISH) in barley genome.It could be estimated that the copy number was about 408-416 for 5S rDNA.In this study,the variability of number of rDNA in genome among different barley species was discussed. |
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| Keywords: | Barley rDNA FISH DNA Fiber-FISH |
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