The chick embryo fibroblast cytosolic DNA complex--a possible cell-cell messenger |
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Authors: | D H Adams C Challen |
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Institution: | Department of Virology, Newcastle upon Tyne, England. |
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Abstract: | 1. The uptake of 3H]thymidine and 3H]uridine labelled DNA-RNA cytosol complex has been studied in chick embryo fibroblast cells. 2. The complex appears to be cleaved into DNA and RNA containing fragments in the recipient cell nucleus: both then enter the cell cytosol fraction, but the RNA fragment in particular is rapidly degraded. 3. Although 3H]thymidine labelled material present in the nucleus co-extracts with bulk nuclear DNA, caesium gradienting shows little or no evidence that integration of host and imported DNA has occurred. 4. It is suggested that the cytosolic/extruded DNA complex may be a "messenger" DNA, capable of the transfer of regulatory information between cells on a transient basis. |
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