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In vitro multiplication of heavy metals hyperaccumulator Thlaspi caerulescens
Authors:J. Xu  Y. X. Zhang  T. Y. Chai  Z. Q. Guan  W. Wei  L. Han  L. Cong
Affiliation:(1) Department of Biology, Graduate University of Chinese Academy of Sciences, Yuquan Rd. 19, Beijing, 100049, China;(2) Department of Bioengineering, China University of Mining and Technology, Xueyuan Rd. 11, Beijing, 100083, China
Abstract:A micropropagation protocol through multiple shoot formation was developed for Thlaspi caerulescens L., one of the most important heavy metals hyperaccumulator plants. In vitro seed-derived young seedlings were used for the initiation of multiple shoots on Murashige and Skoog (MS) medium with combinations of benzylaminopurine (BA; 0.5–1.0 mg dm−3), naphthaleneacetic acid (NAA; 0–0.2 mg dm−3), gibberellic acid (GA3; 0–1.0 mg dm−3) and riboflavin (0–3.0 mg dm−3). The maximum number of shoots was developed on medium containing 1.0 mg dm−3 BA and 0.2 mg dm−3 NAA. GA3 (0.5 mg dm−3) in combination with BA significantly increased shoot length. In view of shoot numbers, shoot length and further rooting rate, the best combination was 1.0 mg dm−3 BA + 0.5 mg dm−3 GA3 + 1.0 mg dm−3 riboflavin. Well-developed shoots (35–50 mm) were successfully rooted at approximately 95 % on MS medium containing 20 g dm−3 sucrose, 8 g dm−3 agar and 1.0 mg dm−3 indolebutyric acid. Almost all in vitro plantlets survived when transferred to pots.
Keywords:micropropagation  growth regulators  riboflavin
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