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白毛茛中生物碱的提取和HPLC分析
引用本文:Holly A Weber Maureen Joseph. 白毛茛中生物碱的提取和HPLC分析[J]. 生物工程学报, 2004, 20(2): 306-308
作者姓名:Holly A Weber Maureen Joseph
作者单位:1.Midwest Research Institute,425 Volker Blvd,Kansas City, MO 64110,USA;2.Agilent Technologies, Inc,2850 Centerville Road,Wilmington, DE 198081610,USA
摘    要:白毛茛根粉末经室温萃取后用HPLC分析其中的生物碱成分,色谱柱为Zorbax Eclipse XDBC18快速分离,该方法可准确测定白毛茛中主要的生物碱,包括小檗碱(berberine)和白毛莨碱(hydrastine)。萃取和HPLC分析还可用于其他几种生物碱的测定,包括氢化小蘖碱(canadine)、白毛莨分碱(hydrastinine)和巴马亭(palmatine),也可以应用于其它含小檗碱的植物根。Eclipse XDBC18快速分离柱采用等梯度分离,所有组分在15min内获得了高的分离度。

关 键 词:生物碱提取, HPLC分析
文章编号:1000-3061(2004)02-0306-03
修稿时间:2003-10-10

Extraction and HPLC Analysis of Alkaloids in Goldenseal
Holly A Weber,Maureen Joseph. Extraction and HPLC Analysis of Alkaloids in Goldenseal[J]. Chinese journal of biotechnology, 2004, 20(2): 306-308
Authors:Holly A Weber  Maureen Joseph
Affiliation:1. Midwest Research Institute,425 Volker Blvd,Kansas City, MO 64110,USA
2. Agilent Technologies, Inc,2850 Centerville Road,Wilmington, DE 19808-1610,USA
Abstract:An ambient extraction of goldenseal root powder followed by HPLC anal ysis of the alkaloids on a Zorbax Rapid Resolution Eclipse XDB-C18 column provi des an accurate method for the determination of key alkaloids in goldenseal, including berberine and hy drastine. The extraction and HPLC analysis can be applied to several other alkal oids, including canadine, hydrastinine, and palmatine, and may be applicable to other berberine-containing plant roots. The Rapid Resolution Eclipse XDB-C18 co lumn is used for an isocratic separation with high resolution of all componentsin under 15 minutes.
Keywords:extraction alkaloids   HPLC analysis
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