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Studies on the development of growth hormone and prolactin cells in the rat pituitary gland by in situ hybridization
Authors:Dr Haruo Nogami  Kaoru Suzuki  Hatsuo Enomoto  Hiroshi Ishikawa
Institution:(1) Département de Physiologie Animale, Institut National de la Recherche Agronomique, Monnaie, Paris, France;(2) Université Pierre et Marie Curie, Paris, France;(3) Jouy en Josas, Paris, France;(4) Département de Physiologie Animale, INRA, F-37380 Monnaie, France
Abstract:Summary An antiserum raised against N-amino-3-propyl melatonin bound to a protein carrier was used to visualize melatonin by immunohistochemistry and to measure melatonin concentration by radioimmunoassay in the pineal gland of intact mink females killed throughout the 24 h cycle and females killed after a bilateral ablation of the cervical superior ganglion. Melatonin immunoreactivity revealed by immunofluorescence or by the peroxidase-antiperoxidase complex was observed in the cytoplasm of presumed pinealocytes of all the females. Circadian changes in pineal melatonin content were not visualized by immunohistochemistry; furthermore, immunoreactivity was also present in the pineal gland of the ganglionectomized females. However, the melatonin content measured by radioimmunoassay was significantly higher in the pineal gland from intact females killed during the night compared with that of intact females killed during the day or of ganglionectomized females. The discrepancy between the results obtained using the two methods may arise because immunohistochemistry can detect very small amounts of melatonin.
Keywords:Melatonin  Immunohistochemistry  Radioimmunoassay  Pineal gland  Mink  Mustela vison
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