Localization of a peptide identified by antibodies to gastrin/CCK in the gut of Cancer magister |
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| Authors: | Frederick W Scalise Brett A Larson Steven R Vigna |
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| Institution: | (1) Department of Biology, University of Oregon, Eugene, USA;(2) Department of Biology, University of Oregon, 97403 Eugene, OR, USA;(3) Present address: Department of Biology, Wesleyan University, Middletown, USA;(4) Present address: Department of Zoology and Cancer Research Laboratory, University of California, Berkeley, USA |
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| Abstract: | Summary A gastric peptide from the Dungeness crab (Cancer magister), extracted and characterized previously (Larson and Vigna 1983b), was localized in the foregut (stomach) of this species by immunocytochemistry using antisera specific for the bioactive carboxy-terminal amino acid sequence common to gastrins and cholecystokinins (CCKs). Immunoreactivity was found in all gastric epithelial cells and in the procuticle. Electron microscopy revealed an absence of peptidergic secretory granules in the gastric epithelial cells. The pattern of immunostaining suggests that the gastric epithelial cells secrete this peptide apically where it is incorporated into the cuticle lining the lumen. Specific immunostaining could not be demonstrated in various neural ganglia or in the hypodermis. The distribution of this peptide is different from that of gastrin/CCK in vertebrates and other invertebrates. This suggests that the crab gastric peptide is sufficiently similar to gastrin/CCK to react with C-terminal specific antisera, but may be anatomically, functionally, and possibly phylogenetically otherwise unrelated. |
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| Keywords: | Crustacea Gastrointestinal hormones Neuropeptides Immunocytochemistry Cuticle Cancer magister |
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