| 大肠杆菌共表达LeGGPS2、LePSY1和crtI基因合成番茄红素 |
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| 引用本文： | 陈吉裕,蒲志群,肖雅文,李翠萍,杜小兵,苏承刚,张兴国. 大肠杆菌共表达LeGGPS2、LePSY1和crtI基因合成番茄红素[J]. 生物工程学报, 2012, 28(7): 823-833 |
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| 作者姓名： | 陈吉裕 蒲志群 肖雅文 李翠萍 杜小兵 苏承刚 张兴国 |
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| 作者单位： | 南方山地园艺学教育部重点实验室 西南大学园艺园林学院, 重庆 400715;南方山地园艺学教育部重点实验室 西南大学园艺园林学院, 重庆 400715;南方山地园艺学教育部重点实验室 西南大学园艺园林学院, 重庆 400715;南方山地园艺学教育部重点实验室 西南大学园艺园林学院, 重庆 400715;南方山地园艺学教育部重点实验室 西南大学园艺园林学院, 重庆 400715;南方山地园艺学教育部重点实验室 西南大学园艺园林学院, 重庆 400715;南方山地园艺学教育部重点实验室 西南大学园艺园林学院, 重庆 400715 |
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| 基金项目： | 国家自然科学基金 (No. 30972008) 资助。 |
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| 摘 要： | 利用大肠杆菌研究番茄红素的合成,不仅可以获得副产物少的高产菌株,而且可以探讨基因或基因簇的功能。文中将番茄LeGGPS2和LePSY1的cDNA序列,及欧文氏菌crtI的编码序列分别添加上核糖体结合位点后,以单独或组合的方式受控于T7启动子和终止子,在大肠杆菌菌株BL21(DE3)中进行表达和诱导番茄红素合成。结果显示,仅T7::crtI-LeGGPS2-LePSY1三价基因共表达时才能合成番茄红素,且将种子液以1∶50接种于含3%蔗糖的LB培养基(pH 6.8)中,于37℃摇8 h左右的对数生长后期加IPTG至80μmol/L,30℃诱导表达5 h的发酵条件下,获得2.124 mg/g DCW的番茄红素。该结果既验证了原核化的番茄LeGGPS2和LePSY1基因及与crtI基因协同作用的功能,又为在番茄质体中建立独立的番茄红素合成途径奠定了基础。
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| 关 键 词： | 大肠杆菌 crtI-LeGGPS2-LePSY1基因共表达 T7/T7RNAP表达系统 番茄红素 |
| 收稿时间： | 2011-12-22 |
Lycopene synthesis via tri-cistronic expression of LeGGPS2, LePSY1 and crtI in Escherichia coli |
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| Jiyu Chen,Zhiqun Pu,Yawen Xiao,Cuiping Li,Xiaobing Du,Chenggang Su and Xingguo Zhang. Lycopene synthesis via tri-cistronic expression of LeGGPS2, LePSY1 and crtI in Escherichia coli[J]. Chinese journal of biotechnology, 2012, 28(7): 823-833 |
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| Authors: | Jiyu Chen Zhiqun Pu Yawen Xiao Cuiping Li Xiaobing Du Chenggang Su Xingguo Zhang |
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| Affiliation: | Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architechture, Southwest University, Chongqing 400715, China;Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architechture, Southwest University, Chongqing 400715, China;Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architechture, Southwest University, Chongqing 400715, China;Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architechture, Southwest University, Chongqing 400715, China;Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architechture, Southwest University, Chongqing 400715, China;Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architechture, Southwest University, Chongqing 400715, China;Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architechture, Southwest University, Chongqing 400715, China |
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| Abstract: | |
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| Keywords: | Escherichia coli tri-cistronic expression of crtI-LeGGPS2-LePSY1 T7/T7RNAP expression system lycopene |
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