| 高通量筛选工具的设计与应用 |
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| 引用本文： | 唐双焱,梁朝宁,蒋培霞.高通量筛选工具的设计与应用[J].生物工程学报,2012,28(7):781-788. |
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| 作者姓名： | 唐双焱 梁朝宁 蒋培霞 |
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| 作者单位： | 中国科学院微生物研究所，北京 100101;中国科学院微生物研究所，北京 100101;中国科学院微生物研究所，北京 100101 |
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| 基金项目： | 国家自然科学基金 (No. 21172095)资助。 |
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| 摘 要： | 定向进化方法作为新兴的高效蛋白质工程手段,其内容包括蛋白质突变体文库的构建和有效突变体的快速筛选。高通量筛选方法是定向进化方法的重要组成部分,是成功获得有效突变体的关键。筛选的突变体数量越多,获得有效突变体的几率越大。以下介绍了目前已经成功应用于或有潜力应用于定向进化改造蛋白质的几种高通量筛选工具。高通量筛选工具的不断设计与开发将推动蛋白质工程领域的技术革新。
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| 关 键 词： | 高通量筛选 定向进化 突变体文库 |
| 收稿时间： | 2012/4/12 0:00:00 |
Design and application of high-throughput screening tools: a review |
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| Shuangyan Tang,Chaoning Liang and Peixia Jiang.Design and application of high-throughput screening tools: a review[J].Chinese Journal of Biotechnology,2012,28(7):781-788. |
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| Authors: | Shuangyan Tang Chaoning Liang and Peixia Jiang |
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| Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | As an efficient and promising protein engineering strategy, directed evolution includes the construction of mutant libraries and screening of desirable mutants. A rapid and high-throughput screening method has played a critical role in the successful application of directed evolution strategy. We reviewed several high-throughput screening tools which have great potential to be applied in directed evolution. The development of powerful high-throughput screening tools will make great contributions to the advancement of protein engineering. |
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| Keywords: | high-throughput screening directed evolution mutant libraries |
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