Location of the LSP-1 Genes in Drosophila Species by IN SITU Hybridization |
| |
Authors: | Brock H W Roberts D B |
| |
Affiliation: | The Genetics Laboratory, Department of Biochemistry, Oxford University, Oxford, United Kingdom OX1 3QU. |
| |
Abstract: | The locations of the larval serum protein one (LSP-1) α, β and γ genes were determined in Drosophila melanogaster and in 14 other species of Drosophila by in situ hybridization to polytene chromosomes. The LSP-1 α gene mapped to bands 11B on the X chromosome, the LSP-1 β gene mapped to bands 21D-E on chromosome 2L, and the LSP-1 γ gene mapped to band 61A in all the melanogaster subgroup species. In eight other species, both the LSP-1 α and β genes mapped to one site on Muller's element E which corresponds to chromosome 3R of D. melanogaster. No hybridization of LSP-1 γ was detected in these eight species. Restriction enzyme digestion and analysis of genomic DNA by filter transfer hybridization confirmed the presence of LSP-1 α-like and β-like genes in seven of these species. These results are discussed with respect to conservation of the chromosomal elements in the genus Drosophila. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|