Rapid In Vitro Multiplication of Dalbergia sissoo Roxb |
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Authors: | V A Chauhan P C Josekutty Y T Jasrai G Prathapasenan |
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Institution: | 1. Department of Botany, Faculty of Science, The M.S University of Baroda, Vadodara, 390 002, India
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Abstract: | Micropropagation of Dalbergia sissoo Roxb was achieved through in vitro proliferation of axillary buds from 30 to 40 years old mature tree. Bud-break was achieved within six days when nodal explants were cultured on MS medium supplemented with kinetin (9.2 μm), indole-3-butyric acid (2.46 μM) apd 6-benzyladenine (13.2 μM). Multiple shoot formation occurred from nodal explants of in vitro raised shoots on MS medium with reduced levels of major salts and kinetin. Roots were Induced within 5 days on in vitro generated shoots on MS medium supplemented with 1-naphthalene acetic acid (0.53 μM) and indole-3-butyric acid (9.8 μM). |
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