Detection of nif Genes in Nitrogen-Fixing Bacterial Isolate from Tomato (Lycopersicon esculentum Mill cv Pusa Ruby) |
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| Authors: | Sukhada Mohandas M S Shailal |
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| Institution: | 2. Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore, 560 089, India
1. Department of Microbiology & Cell Biology, Indian Institute of Science, Bangalore, 560 012, India
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| Abstract: | Nitrogen-fixing bacterial isolate from the intercellular spaces of tomato root cortical cells was studied for the location of nif genes on the chromosomal or plasmid DNA. The bacterial isolate showed two plasmids of approximate molecular sizes of 220 and 120 kb. Klebsiella pneumoniae nif HDK probe hybridized with the chromosomal DNA and not with the plasmid DNA thereby showing that nif genes are localised on the chromosomal DNA. |
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