Molecular cloning of a new endoglucanase gene from Clostridium cellulolyticum and its expression in Escherichia coli |
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| Authors: | Seigo Shima Yasuo Igarashi Tohru Kodama |
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| Institution: | (1) Department of Biology, Abiko Research Laboratory, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko-city, 270-11 Chiba, Japan;(2) Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan |
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| Abstract: | Summary Two genes coding for endoglucanase activity in Clostridium cellulolyticum were cloned and expressed in Escherichia coli by using plasmid pUC18. The sizes of two fragments harbouring endoglucanase genes are 4.4 kb and 2.0 kb, respectively. The 2.0-kb fragment was identical with a reported DNA fragment encoding an endoglucanase of C. cellulolyticum. The 4.4-kb fragment was obtained first in this study. Deletion analysis showed that a 1.3-kb portion of the 4.4-kb fragment is necessary for the endoglucanase expression by its own promoter. The 4.4-kb fragment hybridized with several different fragments of the genomic DNA in C. cellulolyticum.Offprint requests to: T. Kodama |
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