3D electron microscopy of biological nanomachines: principles and applications |
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| Authors: | C. O. S. Sorzano S. Jonic M. Cottevieille E. Larquet N. Boisset S. Marco |
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| Affiliation: | (1) Bioengineering Lab, Escuela Politécnica Superior, Univ. San Pablo CEU, Campus Urb, Montepríncipe s/n, 28668 Boadilla del Monte, Madrid, Spain;(2) IMPMC, Université Pierre & Marie Curie, Campus BOUCICAUT, 140, rue de Lourmel, 75015 Paris, France;(3) Institut Curie, Section Recherche, Laboratoire d’Imagerie Intégrative, Centre Universitaire d’Orsay, 91405 Orsay Cedex, France;(4) INSERM U759, Centre Universitaire d’Orsay, Bat 112, 91405 Orsay Cedex, France |
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| Abstract: | Transmission electron microscopy is a powerful technique for studying the three-dimensional (3D) structure of a wide range of biological specimens. Knowledge of this structure is crucial for fully understanding complex relationships among macromolecular complexes and organelles in living cells. In this paper, we present the principles and main application domains of 3D transmission electron microscopy in structural biology. Moreover, we survey current developments needed in this field, and discuss the close relationship of 3D transmission electron microscopy with other experimental techniques aimed at obtaining structural and dynamical information from the scale of whole living cells to atomic structure of macromolecular complexes. Presented at the joint biannual meeting of the SFB-GEIMM-GRIP, Anglet France, 14–19 October, 2006. |
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