Leucine improves the component of isovalerylspiramycins for the production of bitespiramycin |
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Authors: | Zhen-Lin Li Yong-Hong Wang Ju Chu Ying-Ping Zhuang Si-Liang Zhang |
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Institution: | (1) State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, People’s Republic of China |
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Abstract: | Bitespiramycin, a group of 4″-O-acylated spiramycins with 4″-O-isovalerylspiramycins as the major components, is produced by recombinant spiramycin-producing strain Streptomyces spiramyceticus harboring a 4″-O-acyltransferase gene from a carbomycin-producing strain S. mycarofaciens 1748. The effects of leucine feeding on the bitespiramycin fermentation, especially the synthesis of isovalerylspiramycin
components, were investigated. The experiment was initially performed in flask culture under the condition of feeding 15.4 mmol/l
of leucine at 72 h fermentation, and the culture without leucine feeding was used as control. When 15.4 mmol/l leucine was
fed at 72 h, 51.3 ± 0.33% total isovalerylspiramycins was recorded compared to 40.9 ± 0.26% under the control condition after
96 h of fermentation. The improvement of total isovalerylspiramycin content was further achieved in 15 l fermentation when
15.4 mmol/l of leucine was supplemented from 65 to 72 h. These results indicated that isovaleryl group derived from leucine
catabolism could act as the precursor of the 4″ side chain of bitespiramycin, which profoundly enhanced the synthesis of isovalerylspiramycins
in the bitespiramycin complex. |
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Keywords: | Bitespiramycin Leucine Component Isovalerylspiramycin Streptomyces |
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