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Introduction of Escherichia coli cells and spheroplasts into Vinca protoplasts
Authors:C Matsui  S Hasezawa  N Tanaka  K Syono
Institution:(1) Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya;(2) Department of Pure and Applied Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, Japan
Abstract:In the presence of 10% polyvinyl alcohol (PVA), Escherichia coli cells or spheroplasts can be easily introduced into Vinca protoplasts by endocytosis. Uptake proceeded quite rapidly; bacterial cells or spheroplasts were found within the cytoplasm of Vinca protoplasts after 10 min of incubation with PVA.
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