| 克雷伯杆菌产1,3-丙二醇关键酶甘油脱水酶的酶活分析方法研究 |
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| 引用本文： | 王勇,诸葛斌,方慧英,毛忠贵,诸葛健. 克雷伯杆菌产1,3-丙二醇关键酶甘油脱水酶的酶活分析方法研究[J]. 生物学杂志, 2009, 26(3): 1-3. DOI: 10.3969/j.issn.1008-9632.2009.03.001 |
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| 作者姓名： | 王勇 诸葛斌 方慧英 毛忠贵 诸葛健 |
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| 作者单位： | 江南大学生物工程学院工业微生物研究中心,无锡,214122 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 有氧条件下,建立了克雷伯杆菌破碎方法及其生产1,3-丙二醇代谢途径中关键酶甘油脱水酶的酶活测定方法。甘油脱水酶酶活测定时在超声时间25min,功率为300w的条件下最适破碎频率为破碎时间1s,停息时间4s;甘油脱水酶酶活测定所用磷酸盐缓冲液的最适浓度为0.045mol/L,最适pH值7.2。甘油脱水酶酶活测定反应的最佳温度为37℃,甘油脱水酶酶活测定反应液的最佳吸收波长为290nm。
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| 关 键 词： | 1,3-丙二醇 克雷伯氏杆菌 甘油脱水酶 酶活 |
Study on assay of key enzyme glycerol dehydratase activity of 1, 3-propanediol production by Klebsiella pneumoniae |
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| WANG Yong,ZHUGE Bin,FANG Hui-ying,MAO Zhong-gui,ZHUGE Jian. Study on assay of key enzyme glycerol dehydratase activity of 1, 3-propanediol production by Klebsiella pneumoniae[J]. Journal of Biology, 2009, 26(3): 1-3. DOI: 10.3969/j.issn.1008-9632.2009.03.001 |
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| Authors: | WANG Yong ZHUGE Bin FANG Hui-ying MAO Zhong-gui ZHUGE Jian |
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| Affiliation: | ( School of Biotechnology, Jiangnan University, The Key Laboratory of Industrial Biotechnology Ministry of Education, Wuxi 214122, China) |
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| Abstract: | Under aerobic conditions, the determination method of key enzyme （glycerol dehydratase） activity of 1, 3-pro- panediol production by Klebsiella pneumoniae was established. The optimal conditions of cell disruption by ultrasonic wave were as follow： disruption time 1 second, intermittent time 4 second on disruption power of 300w and ultrasonic time of 25min. Properties of glycerol dehydratase were also studied. Result showed that the optimal concentration of buffer phosphate was 0. 045mol/L, the optimal pH of buffer phosphate was 7.2 and the optimal reactive temperature was 37.2℃. The optimal absorption wavelength of reaction liquor in assay of glycerol dehvdratase activitv is 290nm. |
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| Keywords: | 1,3-propanediol Klebsiella pneumoniae glycerol dehydratase enzyme activity |
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