Regulated genes in mesenchymal stem cells and gastric cancer |
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| Authors: | Shihori Tanabe Kazuhiko Aoyagi Hiroshi Yokozaki Hiroki Sasaki Division of Safety Information on Drug,Food Chemicals,National Institute of Health Sciences,Setagayaku, Tokyo,-,Japan |
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| Affiliation: | Shihori Tanabe;Kazuhiko Aoyagi;Hiroshi Yokozaki;Hiroki Sasaki;Division of Safety Information on Drug, Food and Chemicals, National Institute of Health Sciences, Setagayaku, Tokyo 158-8501, Japan;Department of Translational Oncology, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan;Department of Pathology, Kobe University of Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan; |
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| Abstract: | AIM: To investigate the genes regulated in mesenchymal stem cells (MSCs) and diffuse-type gastric cancer (GC), gene expression was analyzed.METHODS: Gene expression of MSCs and diffuse-type GC cells were analyzed by microarray. Genes related to stem cells, cancer and the epithelial-mesenchymal transition (EMT) were extracted from human gene lists using Gene Ontology and reference information. Gene panels were generated, and messenger RNA gene expression in MSCs and diffuse-type GC cells was analyzed. Cluster analysis was performed using the NCSS software.RESULTS: The gene expression of regulator of G-protein signaling 1 (RGS1) was up-regulated in diffuse-type GC cells compared with MSCs. A panel of stem-cell related genes and genes involved in cancer or the EMT were examined. Stem-cell related genes, such as growth arrest-specific 6, musashi RNA-binding protein 2 and hairy and enhancer of split 1 (Drosophila), NOTCH family genes and Notch ligands, such as delta-like 1 (Drosophila) and Jagged 2, were regulated.CONCLUSION: Expression of RGS1 is up-regulated, and genes related to stem cells and NOTCH signaling are altered in diffuse-type GC compared with MSCs. |
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| Keywords: | Mesenchymal stem cells Gastric cancer Stem cells Gene Epithelial-mesenchymal transition |
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