Two major pathways of zinc(II) acquisition by human placental syncytiotrophoblast

Uptake of zinc into placental villous syncytiotrophoblast is the first step in its transfer from mother to fetus. To help characterise physiologically significant pathways of zinc accumulation by these cells, we incubated cultured layers of syncytiotrophoblast cells derived from human near-term placental tissue with serum ultrafiltrate (containing the zinc complexed with low molecular mass serum constituents), dialysed serum (containing the zinc bound to the serum proteins) and whole serum, each of whose endogenous zinc was tracer-labelled with ⁶⁵Zn(II). Zinc label from both fractions of serum readily entered a rapidly labelled EDTA-sensitive cellular compartment, probably representing zinc bound to the outside cell surface and in accumulative fashion, an EDTA-resistant compartment, probably consisting largely of internalised cellular zinc. Movement of zinc into the EDTA-resistant pool was strongly temperature-dependent and did not occur via the EDTA-sensitive pool from either serum source. Transfer of zinc from the low molecular mass serum fraction into the EDTA-resistant pool was saturable, the concentration giving half-maximal rate being 1.2 m?mol/l nonprotein-bound zinc. No nonsturable component was detected. Zinc from the serum protein-bound fraction entered by a saturable component, already saturated at physiological total protein-bound zinc concentration, and by an apparently nonsaturable component, not appreciably accounted for by nonspecific fluid-phase endocytosis. The results show that zinc is acquired by placental syncytiotrophoblast from the low molecular mass serum zinc pool probably by a carrier-mediated process, and at least as importantly, from the zinc bound to serum protein, possibly by an endocytic mechanism. © 1995 Wiley-Liss, Inc. © 1995 Wiley-Liss, Inc.