Crystallization of the complex of human IFN-γ and the extracellular domain of the IFN-γ receptor |
| |
| Authors: | Christiane Chi ne,Michael Fountoulakis,Heinz D beli,Brigitte D'Arcy,Fritz Winkler,Allan D'Arcy |
| |
| Affiliation: | Christiane Chiène,Michael Fountoulakis,Heinz Döbeli,Brigitte D'Arcy,Fritz Winkler,Allan D'Arcy |
| |
| Abstract: | A complex of human interferon-γ (IFN- γ) with the soluble extracellular domain of the IFN- γ receptor α-chain (IFN-γ-R) has been crystallised. Crystals of the complex were grown using PEG 4000 as the precipitating agent in the presence of β-octyl glucoside. The receptor-ligand complex crystallizes in a monoclinic space group and diffracts to about 3.0 Å resolution. Isomorphous crystals have been obtained with complex containing selenomethionine and cysteine mutants of IFN-γ, which may facilitate the ongoing X-ray structure determination. © 1995 Wiley-Liss, Inc. |
| |
| Keywords: | IFN-γ receptor cytokines crystallization selenomethionine cysteine mutants |
|
|
