A Flow Cytometric Assay Reveals a Suppression of Phagocytosis by Rabbit Defensin NP-3A in Mouse Peritoneal Macrophages |
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| Authors: | Mitsuyuki Ichinose Masashi Sawada |
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| Abstract: | Phagocytosis of fluorescein isothiocyanate (FITC)-labeled polystyrene microparticles by peritonea] macrophages from thioglycollate-elicited mice was examined by means of flow cytometry (FCM). This assay revealed that rabbit defensin NP-3A suppressed the phagocytosis in a dose-dependent manner. The present results suggest that NP-3A released from neutrophils is one of the mediators which modulates the activity of macrophages in response to infection. |
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| Keywords: | macrophages phagocytosis flow cytometry NP-3A |
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