CO2 is the first species formed upon CO oxidation by CO dehydrogenase from Pseudomonas carboxydovorans |
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Authors: | Satoshi Futo Ortwin Meyer |
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Affiliation: | (1) Institut für Mikrobiologie der Georg-August-Universität Göttingen, Grisebachstr. 8, D-3400 Göttingen, Federal Republic of Germany;(2) Present address: Central Laboratory, Nippon Flour Mills Co., Ltd., 2114-2, Nurumizu, Atsugi, 243 Kanagawa, Japan |
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Abstract: | The active species of CO2, i.e. CO2 or HCO3-, formed in the CO dehydrogenase reaction was determined using the pure enzyme from the carboxydotrophic bacterium Pseudomonas carboxydovorans. Employing an assay system similar to that used to test for carbonic anhydrase, data were obtained which are quite compatible with those expected if CO2 is the first species formed. In addition, carbonic anhydrase activity was not detected in P. carboxydovorans. |
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Keywords: | Carbon monoxide Carbon dioxide CO dehydrogenase Carbonic anhydrase Pseudomonas carboxydovorans |
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