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CO2 is the first species formed upon CO oxidation by CO dehydrogenase from Pseudomonas carboxydovorans
Authors:Satoshi Futo  Ortwin Meyer
Affiliation:(1) Institut für Mikrobiologie der Georg-August-Universität Göttingen, Grisebachstr. 8, D-3400 Göttingen, Federal Republic of Germany;(2) Present address: Central Laboratory, Nippon Flour Mills Co., Ltd., 2114-2, Nurumizu, Atsugi, 243 Kanagawa, Japan
Abstract:The active species of ldquoCO2rdquo, i.e. CO2 or HCO3-, formed in the CO dehydrogenase reaction was determined using the pure enzyme from the carboxydotrophic bacterium Pseudomonas carboxydovorans. Employing an assay system similar to that used to test for carbonic anhydrase, data were obtained which are quite compatible with those expected if CO2 is the first species formed. In addition, carbonic anhydrase activity was not detected in P. carboxydovorans.
Keywords:Carbon monoxide  Carbon dioxide  CO dehydrogenase  Carbonic anhydrase  Pseudomonas carboxydovorans
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