CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles |
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Authors: | Donoghue Pamela M McManus Ciara A O'Donoghue Niaobh M Pennington Stephen R Dunn Michael J |
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Institution: | Proteome Research Centre, UCD Conway Institute of Biomolecular and Biomedical Research, Dublin, Ireland. |
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Abstract: | The development of ECL-Plex CyDye-conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of an individual protein species by combining cyanine CyDye pre-labelled proteins and antibody immunoblotting. This technique proposes to revolutionise both 2-D immunoprobing and protein confirmation following MS analysis. |
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Keywords: | Co‐detection CyDye immunoblotting ECL‐Plex Heart Western blotting |
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