| 嗜杀酿酒酵母毒素蛋白及其杀伤质粒的研究 |
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| 引用本文： | 秦玉静 高东. 嗜杀酿酒酵母毒素蛋白及其杀伤质粒的研究[J]. 微生物学报, 2000, 40(1): 105-107 |
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| 作者姓名： | 秦玉静 高东 |
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| 作者单位： | 1. 中国科学院化学研究所
2. 山东大学微生物系,济南,250100 |
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| 摘 要： | Killer toxin from %Saccharomyces cerevisiae% SK was isolated by ultrafiltration of culture supernatants and purified by poly(ethylene glycol). The toxin migrates as one single protein band on SDS-PAGE and its molecular weight is 15kD. The SK toxin has the greatest lethal effect on the sensitive yeast strain in the lat lag phase. Extraction and purification of killer heretity factor(dsRNA) from SK found that M dsRNA plasmid and L dsRNA plasmid have different molecular lengths being 1.7kb and 4.0kb.
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| 关 键 词： | 酿酒酵母 毒素蛋白 杀伤质粒 |
| 文章编号： | 0001-6209(2000)01-0105-07 |
| 修稿时间： | 1999-02-01 |
RESEARCH OF TOXIN AND PLASMIDS OF SACCHAROMYCES CEREVISIAE |
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| Qin Yujing,Gao Dong. RESEARCH OF TOXIN AND PLASMIDS OF SACCHAROMYCES CEREVISIAE[J]. Acta microbiologica Sinica, 2000, 40(1): 105-107 |
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| Authors: | Qin Yujing Gao Dong |
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| Affiliation: | Qin Yujing,Gao Dong;(Department of Microbiology, Shandong University, Jinan250100) |
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| Abstract: | Killer toxin from $accharomyces cerevisiae SK was isolated by ultrafiltration of culture supernatants and purified by poly(ethylene glycol). The toxin migrates as one single protein band on SDS-PAGE and its molecular weight is 15kD. The SK toxin has the greatest lethal effect on the sensitive yeast strain in the lat-lag phase. Extraction and purification of killer heretity factor(dsRNA) from SK found that M-dsRNA plasmid and L-dsRNA plasmid have different molecular lengths being 1.7kb and 4.0kb. |
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| Keywords: | Saccharomyces cerevisiae Toxin Killer plasmid |
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