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应用SRAP标记分析福瑞鲤及其原始亲本的遗传结构
引用本文:曲疆奇,毕滢佳,董在杰,张清靖,胡一丞,袁新华.应用SRAP标记分析福瑞鲤及其原始亲本的遗传结构[J].动物学杂志,2011,46(5):120-125.
作者姓名:曲疆奇  毕滢佳  董在杰  张清靖  胡一丞  袁新华
作者单位:1. 南京农业大学无锡渔业学院 无锡214081;北京市水产科学研究所 北京100068
2. 南京农业大学无锡渔业学院 无锡214081
3. 南京农业大学无锡渔业学院 无锡214081;中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种和养殖生物学重点开放实验室 无锡214081
4. 北京市水产科学研究所 北京100068
基金项目:现代农业产业技术体系建设专项资金项目
摘    要:摘要:应用SRAP分子标记对建鲤(Cyprinus carpio var. jian)、黄河鲤(C.c.haematopterus)和福瑞鲤(FFRC Strain Common Carp,C.carpio)进行遗传结构分析。结果显示,筛选出的10个多态性较好的引物组合共扩增出110个位点,其中多态性位点92个,平均多...

关 键 词:  SRAP  群体  遗传结构
收稿时间:2011/1/26 0:00:00
修稿时间:2011/6/30 0:00:00

Analysis of Genetic Structure of FFRC Strain Common Carp (Cyprinus carpio) and Its Original Parents by SRAP Markers
QU Jiang-Qi,BI Ying-Ji,DONG Zai-Jie,ZHANG Qing-Jing,HU Yi-Cheng and YUAN Xin-Hua.Analysis of Genetic Structure of FFRC Strain Common Carp (Cyprinus carpio) and Its Original Parents by SRAP Markers[J].Chinese Journal of Zoology,2011,46(5):120-125.
Authors:QU Jiang-Qi  BI Ying-Ji  DONG Zai-Jie  ZHANG Qing-Jing  HU Yi-Cheng and YUAN Xin-Hua
Institution:Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081; Beijing Fisheries Research Institute, Beijing 100068, China;Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081;Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081; Key Open Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081;Beijing Fisheries Research Institute, Beijing 100068, China;Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081;Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081; Key Open Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081
Abstract:SRAP markers were used to amplify and analyze the genomic DNA of three Common Carp populations including Jian Carp (Cyprinus carpio var. jian), Huanghe Carp (C.c.haematopterus), and the FFRC Strain Common Carp (C.carpio). Of the 110 bands amplified by 10 primer combinations,92 (83.6%) were polymorphic.The average of polymorphism information content (PIC) was 0.256.The highest and lowest Nei's diversity indexes were found in FFRC Strain Common Carp population (0.233) and Jian Carp population (0.205),respectively.The average Shannon's indexes were 0.332,0.298 and 0.352,respectively in three populations.As to the proportion of polymorphic loci,the most diverse group examined in our work was the FFRC Strain Common Carp population (71.2%),and the least was Huanghe Carp population (65.2%).The results indicate rich polymorphism information content and large genetic diversity in the FFRC Strain Common Carp population.The genetic distance and genetic identity results also suggest that the FFRC Strain Common Carp inherits more genetic material from Jian Carp than from Huanghe Carp.
Keywords:Common Carp (Cyprinus carpio)   SRAP markers  Populations  Genetic structure
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