|
|||||
|
|
| 乌鳢和斑鳢微卫星制备及其对自交与杂交F1代的初步鉴定 | |
| 引用本文： | 朱树人,李忠全,冯晓宇,谢楠,朱林,李家乐.乌鳢和斑鳢微卫星制备及其对自交与杂交F1代的初步鉴定[J].动物学杂志,2011,46(4):1-7. |
| 作者姓名： | 朱树人 李忠全 冯晓宇 谢楠 朱林 李家乐 |
| 作者单位： | 1. 上海海洋大学,省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 2. 杭州市农业科学研究院,杭州,310024 3. 上海海洋大学,省部共建水产种质资源发掘与利用教育部重点实验室,上海201306;上海市高校水产养殖学E研究院,上海,201306 |
| 基金项目： | 杭州市科技局重大科技创新项目(No.20083212A04),上海市水产养殖重点学科建设项目(No.Y1101) |
| 摘 要： | 通过磁珠富集法分离了乌鳢(Channa argus)和斑鳢(C.maculate)的微卫星DNA序列,根据这些微卫星DNA序列的两翼序列,采用Primer 3.0或Primer 5.O软件设计出乌鳢与斑鳢的微卫星引物各31对.用62对微卫星引物对乌鳢和斑鳢自交与杂交F1代的4个组合进行扩增,10对引物无扩增产物,其余5... |
| 关 键 词： | 乌鳢 斑鳢 杂交子代 微卫星 鉴定 |
| 收稿时间： | 2010/11/4 0:00:00 |
| 修稿时间： | 3/3/2011 12:00:00 AM |
Isolation of Microsatellite Makers for Channa argus and C.maculate and Primary Identification of C.argus, C.maculate and Their Reciprocal Hybrids |
|
| ZHU Shu-Ren,LI Zhong-Quan,FENG Xiao-Yu,XIE Nan,ZHU Lin and LI Jia-Le.Isolation of Microsatellite Makers for Channa argus and C.maculate and Primary Identification of C.argus, C.maculate and Their Reciprocal Hybrids[J].Chinese Journal of Zoology,2011,46(4):1-7. | |
| Authors: | ZHU Shu-Ren LI Zhong-Quan FENG Xiao-Yu XIE Nan ZHU Lin and LI Jia-Le |
| Institution: | Shanghai Ocean University, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 201306;Institute of Fisheries, Hangzhou Academy of Agricultural Science, Hangzhou 310024;Institute of Fisheries, Hangzhou Academy of Agricultural Science, Hangzhou 310024;Institute of Fisheries, Hangzhou Academy of Agricultural Science, Hangzhou 310024;Shanghai Ocean University, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 201306;Shanghai Ocean University, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 201306;Aquaculture Division, E-Institute of Shanghai Universities, Shanghai 201306, China |
| Abstract: | In the present study,microsatellite DNA was isolated and enriched from the genomes of Channa argus and C.maculate by magnetic beads.Sixty-two pairs of microsatellite primers of C.argus and C.maculate were designed based on the flanking sequence of the microsatellite loci with software Primer 3.0 or Primer 5.0.Ten primer pairs failed to amplify.Among the 52 primer pairs which could effectively amplify products,49 universal primer pairs could effectively amplify in F1 generation.Loci CHA16 showed monomorphic in C.maculate and polymorphic in C.argus and loci CHM23 was opposite.In addition,CHM24 showed monomorphic in C.argus and failed to show in C.maculae.Moreover,22 specific loci between C.argus and C.maculate were screened out,and 18 specific loci could identify C.argus,C.maculate and the reciprocal hybrids alone effectively.Combination of the other 4 specific loci also effectively identified C.argus,C.maculate and the reciprocal hybrids.However,the reciprocal hybrids C.argus(♀)×C.maculata(♂),C.maculata(♀)×C.argus(♂)]could not be identified in this research. |
| Keywords: | Channa argus C maculate Hybrid Microsatellite Identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《动物学杂志》浏览原始摘要信息 | |
| 点击此处可从《动物学杂志》下载免费的PDF全文 | |