Intracellular Bacterial Symbionts of Aphids Possess Many Genomic Copies per Bacterium |
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Authors: | Kanako Komaki Hajime Ishikawa |
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Affiliation: | (1) Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan, JP |
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Abstract: | Although Buchnera, the endosymbiotic bacteria of aphids, are close relatives of Escherichia coli, their genome size is only a seventh that of E. coli. In this study, we estimated the genomic copy number of Buchnera by dot-blot hybridization and fluorimetry using a video-intensified microscope photon-counting system and obtained convincing evidence that each cell of these bacteria contains an average of 120 genomic copies. Thus, the Buchnera symbiont, with many copies of a small-sized genome, is reminiscent of cell organelles such as mitochondria and chloroplasts. Received: 25 November 1998 / Accepted: 25 December 1998 |
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Keywords: | :Buchnera— Pea aphid — Symbiosis — Genomic copy number — Dot-blot analysis — Microscope photon-counting system — Polyploidy |
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