Characterization of a novel methionine sulfoxide reductase A from tomato (Solanum lycopersicum ), and its protecting role in Escherichia coli |
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Authors: | Dai Changbo Singh Naresh Kumar Park Myungho |
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Affiliation: | Department of Mechanical Engineering, College of Biomedical Science, Kangwon National University, Samcheok 245-711, Korea. |
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Abstract: | Methionine sulfoxide reductase A (MSRA) is a ubiquitous enzyme that has been demonstrated to reduce the S enantiomer of methionine sulfoxide (MetSO) to methionine (Met) and can protect cells against oxidative damage. In this study, we isolated a novel MSRA (SlMSRA2) from Micro-Tom (Solanum lycopersicum L. cv. Micro-Tom) and characterized it by subcloning the coding sequence into a pET expression system. Purified recombinant protein was assayed by HPLC after expression and refolding. This analysis revealed the absolute specificity for methionine-S-sulfoxide and the enzyme was able to convert both free and protein-bound MetSO to Met in the presence of DTT. In addition, the optimal pH, appropriate temperature, and Km and Kcat values for MSRA2 were observed as 8.5, 25oC, 352 ± 25 μM, and 0.066 ± 0.009 S(-1), respectively. Disk inhibition and growth rate assays indicated that SlMSRA2 may play an essential function in protecting E. coli against oxidative damage. |
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