A nonfibrillar form of the fusogenic prion protein fragment [118-135] induces apoptotic cell death in rat cortical neurons |
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Authors: | Pillot T Drouet B Pinçon-Raymond M Vandekerckhove J Rosseneu M Chambaz J |
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Institution: | INSERM U-505, Institut des Cordeliers, Paris, France. Laboratorium voor Lipoproteine Chemie, Universiteit Gent, Gent, Belgium. Jean.Chambaz-u505@bhdc.jussieu.fr |
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Abstract: | Neuronal loss is a salient feature of prion diseases. However, its cause and mechanism, particularly its relationship with the accumulation and precipitation of the pathogenic, protease-resistant isoform PrP(Sc) of the cellular prion protein PrP(C), are still an enigma. Several studies suggest that neuronal loss could occur through a process of programmed cell death, which is consistent with the lack of inflammation in these conditions. By analogy with the pathological events occurring during the development of Alzheimer's disease, controversies still exist regarding the relationship between amyloidogenesis, prion aggregation, and neuronal loss. We recently demonstrated that a prion protein fragment (118-135) displayed membrane-destabilizing properties and was able to induce, in a nonfibrillar form, the fusion of unilamellar liposomes. To unravel the mechanism of prion protein neurotoxicity, we characterize the effects of the human Pr118-135] peptide on rat cortical neurons. We demonstrate that low concentrations of the Pr118-135] peptide, in a nonfibrillar form, induce a time- and dose- dependent apoptotic cell death, including caspase activation, DNA condensation, and fragmentation. This toxicity might involve oxidative stress, because antioxidant molecules, such as probucol and propyl gallate, protect neurons against prion peptide toxicity. By contrast, a nonfusogenic variant Pr118-135, 0 degrees ] peptide, which displays the same amino acid composition but several amino acid permutations, is not toxic to cortical neurons, which emphasizes the critical role of the fusogenic properties of the prion peptide in its neurotoxicity. Taken together, our results suggest that the interaction between the Pr118-135] peptide and the plasma membrane of neurons might represent an early event in a cascade leading to neurodegeneration. |
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Keywords: | Prion protein Synthetic peptide Apoptosis Neurotoxicity Cortical primary neurons |
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