Cloning and expression of the <Emphasis Type="SmallCaps">l</Emphasis>-1-amino-2-propanol dehydrogenase gene from <Emphasis Type="Italic">Rhodococcus erythropolis</Emphasis>, and its application to double chiral compound production |
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Authors: | M Kataoka T Ishige N Urano Y Nakamura E Sakuradani S Fukui S Kita K Sakamoto S Shimizu |
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Institution: | Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan, kataoka@kais.kyoto-u.ac.jp. |
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Abstract: | The gene encoding NADP(+)-dependent L: -1-amino-2-propanol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 was cloned and sequenced. A 780-bp nucleotide fragment was confirmed to be the gene encoding AADH by agreement of the N-terminal and internal amino acid sequences of the purified AADH. The gene (aadh) codes a total of 259 amino acid residues, and the deduced amino acid sequence shows similarity to several short-chain dehydrogenase/reductase family proteins. An expression vector, pKKAADH, which contains the full length aadh was constructed. Escherichia coli cells possessing pKKAADH exhibited a 10.4-fold increase in specific activity as to catalysis of the reduction of (S)-1-phenyl-2-methylaminopropan-1-one (MAK), as compared with that of R. erythropolis MAK154 induced by 1-amino-2-propanol (1 mg/ml). Coexpression of aadh with a cofactor regeneration enzyme (glucose dehydrogenase) gene was also performed, and a system for sufficient production of d-pseudoephedrine from racemic MAK was constructed. |
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Keywords: | Rhodococcus erythropolis Asymmetric reduction l-1-amino-2-propanol dehydrogenase" target="_blank">l-1-amino-2-propanol dehydrogenase Ephedrine |
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