Analysis of nucleotide substitutions and amino acid conservation in theDrosophila Adh genomic region |
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Authors: | R. Albalat G. Marfany R. Gonzàlez-Duarte |
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Affiliation: | (1) Department de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain |
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Abstract: | The homologous genomic region that contains two paralogous genes,Adh andAdh-dup, was compared in severalDrosophila species. Sequences were analyzed as follows: a) At the nucleotide level, Ka and Ks values were determined for each pair of species. Ka-Adh and Ka-Adh-dup are not significantly different. However, Ks-Adh values are significantly lower than Ks-Adh-dup, which are more variable. In agreement with other reports, lower Ks values forAdh correlate with a high level of gene expression and relatively high percentage of G+C content in the third codon position, while the opposite applies toAdh-dup. b) At the protein level, amino acid comparisons reveal conserved regions shared by ADH and ADH-DUP, which have been assigned to known functional domains. Key residues for dehydrogenasic function are also found in ADH-DUP, thus pointing to a dehydrogenase activity for ADH-DUP, albeit very different from that of ADH. |
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Keywords: | Adh Adh-dup Drosophila molecular clock nucleotide substitution rates |
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