Phytosulfokine stimulates somatic embryogenesis in Cryptomeria japonica |
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Authors: | Igasaki Tomohiro Akashi Noriko Ujino-Ihara Tokuko Matsubayashi Yoshikatsu Sakagami Youji Shinohara Kenji |
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Affiliation: | Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute (FFPRI), Tsukuba, 305-8687 Japan. iga@ffpri.affrc.go.jp |
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Abstract: | Phytosulfokine (PSK), which has been identified as a plant growth factor, had a dramatic stimulatory effect on the formation of somatic embryos of sugi (Cryptomeria japonica) in the presence of polyethylene glycol. The resultant somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots, and most of the seedlings grew normally. A cDNA clone for the precursor to the PSK peptide of C. japonica was identified in an expressed sequence tags database. Our results support the existence of a PSK signaling pathway in C. japonica. |
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