Cryopreservation and long-term storage of pear germplasm |
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Authors: | Barbara M. Reed Jeanine Denoma Jie Luo Yongjian Chang Leigh Towill |
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Affiliation: | (1) USDA-ARS National Clonal Germplasm Repository, 33447 Peoria Road, 97333-2521 Corvallis, Oregon;(2) Department of Horticulture, Oregon State University, 97331 Oregon, Corvallis;(3) USDA-ARS National Seed Storage Laboratory, 1111 South Mason Street, 80521 Fort Collins, Colorado |
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Abstract: | Summary Germplasm collections of vegetatively propagated crops are usually maintained as plants in fields or potted in greenhouses or screened enclosures. Safety duplication of these collections, as duplicate plants or separate collections, is costly and requires large amounts of space. Cryopreservation techniques which were recently developed for long-term storage of pear germalasm may offer an efficient alternative to conventional germplasm collection maintenance. Pear (Pyrus L.) germplasm may now be stored as seeds (species), dormant buds or pollen from field-grown trees, or shoot tips fromin vitro-grown plants (cultivars). Pear germplasm may now be cryopreserved and stored for long periods (> 100 yr) utilizing slow-freezing or vitrification ofin vitro-grown shoot-tips. Dormant bud freezing, pollen, and seed cryopreservation of other lines are being developed to complete the base collection forPyrus. This cryopreserved collection provides base (long-term) storage for the field-grown pear germplasm collection at the National Clonal Germplasm Repository, Corvallis, Oregon. Based on a presentation at the 1997 Congress on In Vitro Biology held in Washington, D.C., June 14–18, 1997. |
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Keywords: | cryopreservation genebank liquid nitrogen storage Pyrus slow freezing vitrification |
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