Experimental model ofKlebsiella infection in mice for studies on mechanisms of local immunity of the respiratory tract |
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Authors: | J Libich J Franěk V Hásková |
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Institution: | (1) Institute of Experimental Medicine, Czechoslovak Academy of Sciences, 120 08 Prague 2 |
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Abstract: | An experimental model in white mice, infected with a mildly virulent strain ofKlebsiella pneumoniae, was elaboratored for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal
space of anaesthetised animals proved to be more suitable than the commonly used method of intranasal infection. The strain
administered by the supralaryngeal route, persisted in the lungs of most mice at approximately equal level 1 d after infection,
in some animals it could be demonstrated even after 2–3 d. Using this model (based on various rates of lung clearance), one
can demonstrate faster elimination of klebsiella after a local (supralaryngeal) than systemic (intraperitoneal) immunization
with a heat-inactivated vaccine, prepared from a homologous strain ofK. pneumoniae. |
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