Binding site of the rat liver specific monoclonal antibody |
| |
Authors: | K Sato T Ikeda Y Osada |
| |
Affiliation: | Research Institute, Daiichi Seiyaku Co., Ltd., Tokyo, Japan. |
| |
Abstract: | A rat liver-specific antigen (RLSA) lost its binding ability to the corresponding monoclonal antibody after treatment with N-glycanase or sialidase, which suggested that the specific binding site might be in a portion of the sugar chain containing sialic acid. The specific antigen reacted with wheat germ agglutinin, lentil lectin, erythroagglutinating phytohemagglutinin and Ricinus communis agglutinin, but not with concanavalin A or peanut agglutinin. These results suggest that the specific antigen has asparagine-linked complex-type sugar chains which might be the binding sites of the monoclonal antibody. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|