Micropropagation of <Emphasis Type="Italic">Pongamia pinnata</Emphasis> through enhanced axillary branching |
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Authors: | Twinkle Sugla Jubilee Purkayastha Shashi Kant Singh Siva Kumar Solleti Lingaraj Sahoo |
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Institution: | (1) Center for Energy, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India;(2) Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India |
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Abstract: | A complete protocol is presented for the first time for the micropropagation of Pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Multiple shoots were induced in vitro from nodal segments through forced axillary branching. Murashige and Skoog (MS) medium supplemented with 7.5 μM benzylaminopurine
(BAP) induced up to 6.8 shoots per node with an average shoot length of 0.67 cm in 12 d. Incorporation of 2.5 μM gibberellic
acid (GA3) in the medium during the first subculture after establishment and initiation of shoot buds significantly improved the shoot
elongation. Single use of GA3 during the first subculture eliminated the need for prolonged culturing on BAP medium. Further use of GA3 in the medium was not useful. Shoot culture was established for at least two subcultures without loss of vigor by repeatedly
subculturing the original cotyledonary node on shoot multiplication medium followed by shoot elongation medium after each
harvest of the newly formed shoots. Thus, from a single cotyledonary node, about 16–18 shoots were obtained in 60 d. Shoots
formed in vitro were rooted on full-strength MS medium supplemented with 1.0 μM indole butyric acid (IBA). Plantlets were successfully acclimated,
established in soil, and transferred to the nursery. |
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Keywords: | Axillary proliferation Biofuel tree Cotyledonary nodes Juvenile explant Micropropagation Pongamia pinnata |
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