Rapid changes of microsatellite flanking sequence in the allopolyploidization of new synthesized hexaploid wheat |
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Authors: | Lianquan?Zhang Email author" target="_blank">Dengcai?LiuEmail author Zehong?Yan Xiujin?Lan Youliang?Zheng Yonghong?Zhou |
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Institution: | Triticeae Research Institute,Sichuan Agricultural University,Dujiangyan 611830,China |
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Abstract: | It was suggested that the rapid changes of DNA sequence and gene expression occurred at the early stages of allopolyploid
formation. In this study, we revealed the microsatellite (SSR) differences between newly formed allopolyploids and their donor
parents by using 21 primer sets specific for D genome of wheat. It was indicated that rapid changes had occurred in the “shock”
process of the allopolyploid formation between tetraploid wheat and Aegilops tauschii. The changes of SSR flanking sequence resulted in appearance of novel bands or disappearance of parental bands. The disappearance
of the parental bands showed much higher frequencies in comparison with that of appearance of novel bands. Disappearance of
the parental bands was not random. The frequency of disappearance in tetraploid wheat was much higher than in Ae. tauschii, i. e. the disappearance frequency in AABB genome was much higher than in D genome. Changes of SSR flanking sequence occurred
at the early stage of F1 hybrid or just after chromosome doubling. From the above results, it can be inferred that SSR flanking sequence region was
very active and was amenable to change in the process of polyploidization. This suggested that SSR flanking sequence probably
had special biological function at the early stage of ployploidization. The rapid and directional changes at the early stage
of polyploidization might contribute to the rapid evolution of the newly formed allopolyploid and allow the divergent genomes
to act in harmony. |
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Keywords: | synthesized hexaploid wheat genome evolution microsatellite allopolyploidization SSR |
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